Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

Deciphering composition and function of the root microbiome of a legume plant

Kyle Hartman, Marcel G. A. van der Heijden, Valexia Roussely-Provent, Jean‐Claude Walser, Klaus Schlaeppi

Microbiome · 2017

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Summary

This study characterises the root bacterial microbiome of red clover (Trifolium pratense), a legume of agricultural significance, combining culture-dependent and molecular methods to identify dominant taxa. Through controlled microcosm inoculation experiments, the authors demonstrate that whilst individual abundant microbiome members can compromise plant growth in isolation, community-level diversity buffers against these negative effects, suggesting functional redundancy and complementarity within the root microbiota.

UK applicability

Red clover is an established forage crop in UK grassland and agroecological systems, making findings directly relevant to UK pasture management and soil health strategies. The demonstration that microbiome diversity alleviates individual pathogenic or growth-inhibitory members has implications for promoting resilience in UK farming systems through microbial community management.

Key measures

Root microbiome taxonomic composition (proportion of Rhizobia and other bacterial genera); bacterial isolate library size and coverage of abundant microbiome members; plant growth outcomes under monoculture and mixed microbial inoculations

Outcomes reported

The study characterised the root bacterial microbiome of Trifolium pratense (red clover) using culture-dependent and independent methods, identifying dominant taxa and their functional roles. Inoculation experiments demonstrated that individual microbiome members can negatively affect plant growth when isolated, but these effects are alleviated in more diverse microbial communities.

Theme
Farming systems, soils & land use
Subject
Soil biology & microbiology
Study type
Research
Study design
Laboratory experimental study with microcosm inoculation trials
Source type
Peer-reviewed study
Status
Published
Geography
International
System type
Laboratory / in vitro
DOI
10.1186/s40168-016-0220-z
Catalogue ID
BFmor3gc43-p9m62j

Topic tags

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