Summary
This study aimed to investigate the effects of varying dietary protein levels on growth performance, meat quality traits, amino acid and fatty acid compositions, and hindgut microbiota in Dezhou donkeys. Eighteen 12-month-old male donkeys, weighing 188 ± 9 kg, were randomly allocated into three groups and fed diets containing 11.03% (LP), 12.52% (MP), and 14.06% (HP) protein. The average daily gain (ADG) was significantly higher (<i>p</i> < 0.05) in the HP and MP groups, while the feed conversion ratio (FCR) was lower (<i>p</i> < 0.05) compared to the LP group. The MP group exhibited superior performance in terms of serum albumin (ALB) and high-density lipoprotein (HDL) levels, as well as protein digestibility (<i>p</i> < 0.05). Improvements in meat tenderness, as well as increased levels
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