Summary
Regenerative agriculture is a multi-faceted approach that aims at transitioning farmers from conventional to sustainable management practices, by increasing biodiversity, functional redundancy, and nutrient cycling efficiency in the soil. This study investigated the effects of three crop rotation systems, wheat after wheat, wheat after medicago, and wheat after canola on the soil fungal and bacterial communities, in a regenerative agriculture system, in the Western Cape, South Africa, following a record-breaking drought (2015–2019). Utilising 16S rRNA and ITS targeted amplicon sequences, soil-geochemical properties, and qPCR analyses, it was found that crop rotations had little significant effect on the alpha-diversity between different crop-rotation systems. Medicago and wheat had the m
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