Pulse Brain · Growing Health Evidence Index
Tier 2 — RCT / large cohortPeer-reviewed

Unravelling the bioavailability of amino acids and bioactive peptides from collagen hydrolysate in coffee in healthy volunteers.

N. Virgilio; C. Schoen; B. van der Steen; A. Kleinnijenhuis; F.L. van Holthoon; S. Vleminckx; C.I.F. Silva; J. Prawitt

Food Research International · 2025

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Summary

This randomised crossover clinical trial investigated whether the polyphenol-rich matrix of coffee affects the absorption of signature amino acids and bioactive peptides derived from bovine collagen hydrolysate in healthy humans. Given the known affinity of dietary polyphenols for proline-rich proteins, the study addressed a practically relevant but previously unresolved question about potential matrix-mediated interference with collagen peptide bioavailability. Findings are likely to inform product formulation guidance for collagen-fortified beverages, though any confirmed attenuation or equivalence of absorption would carry direct implications for consumer supplementation practice.

UK applicability

The findings are broadly applicable to UK consumers and practitioners given the widespread and growing use of collagen hydrolysate supplements, including in coffee-based products; no specific geographical barrier limits the relevance of the pharmacokinetic data to a UK population.

Key measures

Plasma amino acid concentrations (hydroxyproline, glycine, proline); plasma bioactive peptide levels (Pro-Hyp, Hyp-Gly, Gly-Pro-Hyp); pharmacokinetic parameters (likely Cmax, Tmax, AUC)

Outcomes reported

The study measured plasma concentrations of hydroxyproline, glycine, and proline, and bioactive peptides (Pro-Hyp, Hyp-Gly, Gly-Pro-Hyp) following ingestion of collagen hydrolysate dissolved in either water or coffee, in healthy adult volunteers.

Theme
Nutrition & health
Subject
Nutrient bioavailability & absorption
Study type
Research
Study design
RCT
Source type
Peer-reviewed study
Status
Published
Geography
Europe
System type
Human clinical
DOI
10.1016/j.foodres.2025.116478
Catalogue ID
NRmo3dpodv-00a

Topic tags

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