Multi-targeted MS-based metabolomics fingerprinting of black and white pepper coupled with molecular networking in relation to their <i>in vitro</i> antioxidant and antidiabetic effects.

Summary

This study applies multi-targeted mass spectrometry-based metabolomics combined with molecular networking to comprehensively characterise the phytochemical profiles of black and white pepper (Piper nigrum). The work correlates the identified metabolite fingerprints — likely including alkaloids such as piperine, flavonoids, and phenolic acids — with quantified in vitro antioxidant and antidiabetic activities. The approach offers a systematic framework for linking specific pepper-derived compounds to functional health-relevant bioactivities, potentially informing future nutraceutical or dietary research.

UK applicability

Pepper is widely consumed in the UK but not domestically grown; findings on its bioactive phytochemical profile are broadly applicable to UK nutritional research, dietary guidance, and functional food development, though clinical translation would require in vivo and human studies.

Key measures

Metabolite identification via MS-based metabolomics; molecular networking outputs; in vitro antioxidant capacity (e.g. DPPH, ABTS assays); antidiabetic enzyme inhibition (e.g. α-glucosidase, α-amylase IC50 values)

Outcomes reported

The study characterised the metabolite profiles of black and white pepper using mass spectrometry-based metabolomics and molecular networking, then evaluated their in vitro antioxidant and antidiabetic (enzyme inhibition) activities. It aimed to identify specific phytochemical constituents responsible for observed bioactivities.

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