Summary
Salt stress poses a significant threat to crop growth. While brassinolide (BR) has been shown to alleviate its adverse effects and modulate plant development, the precise mechanism underlying BR-induced salt tolerance in rice remains unclear. In this study, the Chaoyouqianhao and Huanghuazhan rice varieties were employed to investigate the effects of BR seed soaking on the seedling phenotype, physiology, transcriptome, and metabolome under salt stress. The results demonstrated that BR treatment significantly enhanced rice plant height, root length, biomass, and antioxidant enzyme activities, while reducing leaf membrane damage, promoting ion homeostasis, and improving the photosynthetic capacity and salt tolerance. The transcriptome analysis revealed that BR regulated the expression of 104
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