Summary
To elucidate the direct effects of biochar on denitrification metabolism at the cellular level, the global response of model denitrifying soil bacterium (<i>Paracoccus denitrificans</i>) to biochar addition was investigated by physiological, proteomic, and metabolomics analyses. The enhancement effect on denitrification was positively correlated with its pyrolysis temperatures (300-500 °C) and dosages (0.1-1%), regardless of precursors [corn straw (CS) or wheat straw). Moreover, the stimulating effect of CS biochar made at 500 °C (CS-500) was mainly attributed to the bulk particles rather than the released soluble compounds. Without direct contact with cells, bulk CS-500 particles might directly modulate the carbon metabolism by the adsorption of extracellular metabolites. Since carbon flu
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