Summary
The organic carbon content of soil (SOC) is considered a key factor for soil health and plays an important role in climate change. Conservation tillage systems promote carbon sequestration and reduce greenhouse gas emissions. A long-term field experiment with different soil tillage practices (conventional tillage—CT; reduced tillage—RT; and no tillage—NT) has been conducted in Prague-Ruzyně (Czech Republic) since 1995. The soil’s organic carbon content, microbial biomass (Cmic), and enzymatic characteristics were evaluated in four-year crop rotation periods from 2005 to 2024. The crop rotation was as follows: winter oil seed rape, winter wheat, pea, and winter wheat. The following soil layers were studied: 0–10, 10–20, and 20–30 cm. Crop residues remained in the field and were incorporated
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