Pulse Brain · Growing Health Evidence Index
Peer-reviewed

Promotion of Cell Proliferation through Inhibition of Cell Autophagy Signalling Pathway by Rab3IP is Restrained by MicroRNA-532-3p in Gastric Cancer

Weihong Guo, Zhaoyu Chen, Zhian Chen, Jiang Yu, Hao Liu, Tuanjie Li, Lin Tian, Hao Chen, Mingli Zhao, Guoxin Li, Yanfeng Hu

Journal of Cancer · 2018

Read source ↗ All evidence

Summary

<b>Background:</b> RAB3A-interacting protein (Rab3IP) is known to be involved in cancer; however, its function during the proliferation of gastric cancer (GC) cells remains unknown. Therefore, this study aimed to explore the potential function of Rab3IP in GC. <b>Methods:</b> The expression of Rab3IP and its clinical pathology value were determined by quantitative real-time PCR and immunohistochemistry. Rab3IP (knockdown and overexpression) and light chain 3 (LC3) lentiviruses were transfected into GC cells, and cell proliferation was measured using cell counting kit-8, plate clone formation, flow cytometry, and tumorigenesis assays. Cell autophagy was measured using a confocal laser scanning microscope and by western blotting. Luciferase reporter assay was performed to analyse the regulat

Source type
Peer-reviewed study
DOI
10.7150/jca.27533
Catalogue ID
SNmoi8o7l4-5ebhqj
Pulse AI · ask about this record

Dig deeper with Pulse AI.

Pulse AI has read the whole catalogue. Ask about this record, its theme, or how the findings apply to UK farming and policy — every answer cites the underlying studies.