Pulse Brain · Growing Health Evidence Index
Peer-reviewed

On the impact of batch effect correction in TCGA isomiR expression data

Susanne Ibing, Birgitta E. Michels, Moritz Mosdzien, Helen R Meyer, Lars Feuerbach, Cindy Körner

NAR Cancer · 2021

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Summary

MicroRNAs (miRNAs) are small non-coding RNAs with diverse functions in post-transcriptional regulation of gene expression. Sequence and length variants of miRNAs are called isomiRs and can exert different functions compared to their canonical counterparts. The Cancer Genome Atlas (TCGA) provides isomiR-level expression data for patients of various cancer entities collected in a multi-center approach over several years. However, the impact of batch effects within individual cohorts has not been systematically investigated and corrected for before. Therefore, the aim of this study was to identify relevant cohort-specific batch variables and generate batch-corrected isomiR expression data for 16 TCGA cohorts. The main batch variables included sequencing platform, plate, sample purity and sequ

Source type
Peer-reviewed study
DOI
10.1093/narcan/zcab007
Catalogue ID
SNmoi8o93j-6qfqi8
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