Summary
Nontuberculous mycobacteria (NTM) are emerging global pathogens. This study aimed to analyze the clinical features, species distribution, and drug resistance profiles of NTM infections in a northern Chinese hospital. A retrospective cohort study included 1769 patients with suspected mycobacterial infection from August 2023 to July 2025. PCR melting curve analysis (PCR-MCA) was used for initial screening, with confirmation by targeted next-generation sequencing (tNGS). Confirmed cases were classified as localized or disseminated disease. Among 60 confirmed NTM cases, 48 were localized (mainly pulmonary) and 12 were disseminated. The predominant species in localized disease were Mycobacterium intracellulare (35.4%) and Mycobacterium abscessus (35.4%), while disseminated cases were primarily
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