Summary
Application of biochar to soil has been recommended as a carbon sequestration approach that can also improve soil physical and chemical properties. The addition of biochar to soil can change the physicochemical properties of the soil, leading to a subsequent modification of the microbial community. However, the long-term implications of these changes remain insufficiently elucidated. Here, we examined soil chemical and biochemical properties of the bulk soil and employed next-generation sequencing techniques to analyze the microbiological properties of both bulk and rhizosphere soils after 10 years of biochar application. Specifically, we compared these properties between soil treated with two doses of biochar, i.e., SB and DB, and untreated soil, i.e., CK. After 10 years, biochar applicat
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