Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

A highly conserved core bacterial microbiota with nitrogen-fixation capacity inhabits the xylem sap in maize plants

Liyu Zhang, Meiling Zhang, Shuyu Huang, Lu‐Jun Li, Qiang Gao, Yin Wang, Shuiqing Zhang, Shaomin Huang, Liang Yuan, Yanchen Wen, Kailou Liu, Xichu Yu, Dongchu Li, Lu Zhang, Xinpeng Xu, Hai‐Lei Wei, Ping He, Wei Zhou, Laurent Philippot, Chao Ai

Nature Communications · 2022

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Summary

This study characterises the highly conserved bacterial microbiota inhabiting maize stem xylem sap across diverse soil types, climatic zones and maize genotypes, revealing selective recruitment of Gammaproteobacteria with elevated nitrogen-fixation capacity. Synthetic communities comprising core diazotrophs and helper bacteria were shown to actively fix nitrogen in planta, contributing approximately 12% of the total nitrogen accumulated in maize stems. The findings suggest that endophytic nitrogen-fixing communities represent an underexploited biological resource for improving crop productivity and nitrogen nutrition.

UK applicability

The study's findings on endophytic nitrogen fixation in maize are potentially relevant to UK cereal production, though regional adaptation would be required given the original research was conducted in China. The principle of harnessing plant-associated diazotrophs could support nitrogen-use efficiency improvements in UK arable systems, but field validation under UK temperate conditions and soil types would be necessary before practical application.

Key measures

Bacterial community composition (16S rRNA sequencing), nitrogenase gene (nifH) abundance, nitrogen fixation contribution via 15N isotopic dilution method, GFP-tagged strain tracking

Outcomes reported

The study identified 25 core bacterial taxa in maize stem xylem sap, enriched in nitrogen-fixing Gammaproteobacteria. Synthetic communities (SynComs) of selected diazotrophs and helper strains contributed 11.8% of total nitrogen accumulated in maize stems through biological nitrogen fixation.

Theme
Farming systems, soils & land use
Subject
Soil biology & microbiology
Study type
Research
Study design
Field trial with microbial isolation, characterisation and experimental validation
Source type
Peer-reviewed study
Status
Published
Geography
China
System type
Arable cereals
DOI
10.1038/s41467-022-31113-w
Catalogue ID
SNmojqlwj8-6u3thl

Topic tags

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