Summary
Soil microorganisms are essential for maintaining the function and health of agricultural ecosystems. However, the responses of microbial communities to long-term changes in land use have been insufficiently explored. Hence, based on a 15 years of field experiments in the northeast Mollisol region of China, we applied the Illumina high-throughput sequencing technology to study the effects of different land use types, including conventional tillage (CT), bare land (BL), no tillage (NT), natural vegetation restoration (NVR), and afforestation (AF), on bacterial communities along the soil profile (0–5 cm, 5–10 cm, 10–20 cm, and 20–30 cm) and co-occurrence networks and identified their relationships with soil physicochemical properties. The findings indicated that the land use type as well as
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