Summary
Introduction Understanding how pasture-crop rotation design influences long-term soil health and microbial dynamics is crucial for sustainable agroecosystems. Pasture-crop rotations may alleviate soil degradation, but their long-term effects or legacy on soil and rhizosphere microbiomes, including potential recruitment of plant-beneficial microorganisms, and their link to crop yield need to be better understood. This study examines how land use intensity and grazed pasture legacy influence soil and rhizosphere prokaryotic diversity, composition, functionality, and crop productivity. Methods A gradient of land use intensities ranging from continuous cropping (CCG), short (SR) and long (LR) pasture-crop rotations, permanent improved pasture (PIP), and natural grassland (NGL) were sampled in
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