Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

Multitargeted metabarcoding and morphological identification reveal practice-specific soil biodiversity outcomes in Mediterranean cropping systems

Lur Epelde, Ninon Delcourt, Jérôme Cortet, Anders Lanzén, Carlos Garbisu, José Luis Lavín, Luigi Paolo D’Acqui, Sara Di Lonardo, Martina Grattacaso, Eliana Tassi, Fatima‐Zahraa El Balghiti, Leila Benidire, Ali Boularbah, Ángeles Prieto-Fernández, Carmen Trasar-Cepeda, Sihem Soufi, Mohamed Allani, Taoufik Bettaieb, Ali Sahli, Ana Sofia Sousa, Helena Moreira, Paula M. L. Castro, Sofia I.A. Pereira

SSRN Electronic Journal · 2025

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Summary

This multi-country field study applied integrated metabarcoding and morphological identification to characterise soil biodiversity outcomes across Mediterranean cropping systems. By combining molecular and traditional approaches, the authors generated a detailed picture of how different farming practices shape soil community structure. The findings are intended to support evidence-based assessment of soil health and farming system sustainability across Mediterranean regions.

Regional applicability

This study was conducted in Mediterranean regions (Spain, France, Morocco, Tunisia, Portugal, Italy) and may have limited direct applicability to United Kingdom soil and climate conditions, which differ substantially in temperature regime, moisture availability, and soil types. However, the methodological approach combining metabarcoding with morphological identification could inform UK soil biodiversity monitoring frameworks.

Key measures

Metabarcoding-derived taxonomic profiles of soil microorganisms and fauna; morphological identification of nematodes and arthropods; biodiversity indices across cropping system types

Outcomes reported

The study characterised soil biodiversity outcomes (microbial, nematode, and arthropod communities) across different Mediterranean cropping systems using combined metabarcoding and morphological identification. Results are presented as practice-specific biodiversity profiles linked to farming management approaches.

Theme
Farming systems, soils & land use
Subject
Soil biology & microbiology
Study type
Research
Study design
Field trial
Source type
Peer-reviewed study
Status
Preprint
Geography
International
System type
Mixed farming
DOI
10.2139/ssrn.5787315
Catalogue ID
SNmomgy9b3-vo5xfq

Topic tags

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