Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

Evolutionary Rewiring of Anthocyanin Biosynthesis Pathway in <i>Poaceae</i>

Najnin Khatun, Aidan Jones, Avery Rahe, Nancy Choudhary, Boas Pucker

bioRxiv (Cold Spring Harbor Laboratory) · 2025

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Summary

This comparative genomic study reveals that anthocyanin biosynthesis pathways in Poaceae grasses have undergone independent evolutionary rewiring, with distinct regulatory and enzymatic components compared to other plant families. An independently evolved ncaMYB lineage controls anthocyanin production in grasses, whilst a derived BZ2 lineage replaced the otherwise conserved TT19/An9 glutathione S-transferase. The authors hypothesise a complex evolutionary history involving ancestral pathway loss at the base of Poaceae, followed by independent regain, and secondary loss in the genus Brachypodium.

Regional applicability

This is a fundamental plant genomics study with no specified geographic conduct location. The findings are globally relevant to understanding grass crop biology and may inform breeding programmes for anthocyanin-enriched grain varieties, though direct application to United Kingdom farming systems requires further trait characterisation in temperate cereal crops.

Key measures

Phylogenetic presence/absence of anthocyanin biosynthesis genes (ANS, arGST/BZ2, ncaMYB); evolutionary lineage classification; gene family composition across Poaceae genera

Outcomes reported

The study characterised patterns of gene presence, absence, and evolutionary lineage replacement in anthocyanin biosynthesis pathways across Poaceae species. The research identified an independently evolved MYB regulatory lineage (ncaMYB) and a BZ2 glutathione S-transferase lineage that replaced the typical TT19/An9 lineage in grasses, with evidence of pathway loss in Brachypodium.

Theme
General food systems / other
Subject
Phytochemicals & bioactive compounds
Study type
Research
Study design
Comparative genomic analysis
Source type
Peer-reviewed study
Status
Preprint
System type
Laboratory / in vitro
DOI
10.1101/2025.09.21.677584
Catalogue ID
SNmomgycxv-p3lpb7

Topic tags

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