Summary
This paper describes development of a semi-automated flow injection analysis (FIA) system for rapid determination of total polyphenol content in legume flours, based on the Folin–Ciocalteu method. The FIA approach achieved significant improvements over conventional batch procedures: higher throughput (20 samples in triplicate within one hour), reduced reagent use, and lower operational complexity, whilst maintaining agreement with reference methodology. This greener, faster analytical platform may facilitate broader nutritional characterisation of legume quality in seed selection and crop evaluation.
Regional applicability
The methodology is geographically neutral and could be adopted by any research institution or quality assurance laboratory in the United Kingdom testing legume flours or seeds. Given the UK's growing interest in plant proteins and legume cultivation as part of sustainable farming and nutrition policy, this analytical tool would support domestic crop characterisation and breeding programmes.
Key measures
Total polyphenol content (TPC) in legume flours; limit of detection (LOD: 4.32 mg L⁻¹); limit of quantification (LOQ: 14.4 mg L⁻¹); relative standard deviation (RSD: 4.4%); analytical throughput (1 sample per minute); reagent consumption
Outcomes reported
The study developed and validated a semi-automated flow injection analysis (FIA) method for measuring total polyphenol content (TPC) in legume flours, achieving high throughput (1 analysis per minute) with lower reagent consumption than conventional methods. The novel FIA method was applied to various legume flour samples and its results were compared with the reference Folin–Ciocalteu procedure.
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