Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

Unlocking plant-microbial interactions in deep Mollisols in the Midwestern US: Linking depth gradients in roots, microbial activity, and soil carbon in agroecosystems

Zoe Pagliaro, Emel Kangi, Justin M. Mathias, J Burke, Rachel Van Allen, Ember M. Morrissey, Yuan Liu, Wendy H. Yang, Edward Brzostek

Biogeochemistry · 2026

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Summary

This field study in mature miscanthus agroecosystems examined how plant-microbial interactions drive soil carbon accumulation with depth in Mollisols to 1 m. Root biomass, enzyme activity, and microbial respiration collectively explained 74% of total soil carbon variability and 38% of mineral-associated organic carbon variability. The results suggest that deep-rooted perennial plants may build persistent soil carbon stocks through reduced priming losses and enhanced microbial necromass production in deeper soil layers.

UK applicability

UK conditions differ substantially from the Midwestern United States climate and Mollisol-Argiudoll soil types studied; direct application would require validation on UK soil series. However, the mechanistic understanding of how perennial rooting depth influences carbon stability could inform UK regenerative agriculture strategies, particularly for deep-rooting species in grassland restoration or agroforestry systems.

Key measures

Fine root biomass, total soil carbon, mineral-associated organic carbon (MAOC), particulate organic carbon (POC), potential enzyme activity (acid phosphatase and n-acetyl-glucosaminidase), microbial respiration, 13C-labelled glucose incorporation and loss

Outcomes reported

The study quantified how fine root biomass, microbial enzyme activity, and microbial respiration correlate with soil carbon stocks (total C, mineral-associated organic carbon, and particulate organic carbon) to 1 m depth in miscanthus agroecosystems. It measured the fate of glucose-derived carbon inputs with depth to assess susceptibility to priming losses and carbon persistence.

Theme
Farming systems, soils & land use
Subject
Soil biology & microbiology
Study type
Research
Study design
Field trial
Source type
Peer-reviewed study
Status
Published
Geography
United States
System type
Regenerative systems
DOI
10.1007/s10533-026-01331-1
Catalogue ID
SNmov0f4ef-jjgzv7

Topic tags

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