Summary
This paper describes the updated INFOGEST consensus static in vitro digestion method, developed by an international network of researchers to harmonise simulation of human gastrointestinal digestion across laboratories. The protocol standardises conditions for the oral, gastric, and small intestinal phases, including specific enzyme sources and activities, pH values, and ionic compositions. It is widely used in food and nutritional research to assess nutrient bioaccessibility and the digestive behaviour of food matrices without reliance on human or animal trials.
UK applicability
The INFOGEST protocol is internationally applicable and is used extensively by UK food scientists and nutritional researchers to evaluate nutrient release and bioaccessibility from diverse food systems. Its adoption supports comparability of UK-based in vitro digestion studies with international literature.
Key measures
Enzyme activity units (U/mL); pH at each digestion phase; electrolyte concentrations (mM); digestion duration (minutes); bioaccessibility of nutrients as proxy outcome
Outcomes reported
The paper presents a consensus-based, updated static in vitro digestion protocol (INFOGEST 2.0), specifying conditions for oral, gastric, and intestinal digestion phases. It reports standardised parameters including enzyme activities, pH, electrolyte composition, and digestion times intended to improve reproducibility across laboratories.
Topic tags
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