Summary
This paper presents a novel analytical method combining optimised sample preparation with LC-MS/MS detection for the rapid and simultaneous determination of seven B-group vitamins in infant formula and related nutritional matrices. The method is reported to offer improved precision and accuracy relative to existing approaches, reducing analytical time whilst maintaining regulatory-grade performance. It is likely to be of direct relevance to food testing laboratories and regulatory compliance work in the nutritional products sector.
UK applicability
Whilst the study is not geographically specific, the validated method is applicable to UK and European food testing laboratories operating under infant formula compositional regulations (e.g., EU Delegated Regulation 2016/127 and retained UK equivalents), where accurate B-vitamin quantification is a regulatory requirement.
Key measures
Precision (repeatability and reproducibility), accuracy (recovery), linearity, limits of detection and quantification for vitamins B1, B2, B3, B5, B6, B7, and B9 (µg per 100 g or per serving)
Outcomes reported
The study developed and validated a combined sample preparation and chromatographic separation method for the simultaneous quantification of vitamins B1, B2, B3, B5, B6, B7, and B9 in infant formula and related nutritional products by liquid chromatography–tandem mass spectrometry (LC-MS/MS).
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