Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

Rapid generation of potent antibodies by autonomous hypermutation in yeast

Alon Wellner, Conor McMahon, Morgan S. A. Gilman, Jonathan R. Clements, Sarah A. Clark, Kianna M. Nguyen, Ming Hua Ho, Vincent J. Hu, Jung-Eun Shin, Jared Feldman, Blake M. Hauser, Timothy M. Caradonna, Laura M. Wingler, Aaron G. Schmidt, Debora S. Marks, Jonathan Abraham, Andrew C. Kruse, Chang C. Liu

Nature Chemical Biology · 2021

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Summary

This paper presents a synthetic biology approach to accelerate antibody discovery by engineering autonomous hypermutation pathways within yeast cells, circumventing the need for animal immunisation or traditional phage display methods. The authors demonstrate rapid generation of diverse, high-affinity antibodies through in vitro evolution, with potential applications in therapeutic antibody development. The work represents an advance in antibody engineering methodology rather than a study of agricultural or nutritional systems.

UK applicability

This laboratory-based synthetic biology research has no direct application to UK farming systems, soil health, or food-production practices. Its relevance to Vitagri's Pulse Brain is tangential unless applied to food-safety antibody development or diagnostic applications in agricultural contexts.

Key measures

Antibody binding affinity, mutation frequency, antibody diversity metrics, functional potency assays

Outcomes reported

The study reports the development and characterisation of a yeast-based platform for autonomous generation of potent antibodies through engineered hypermutation pathways. Key outcomes include demonstration of antibody diversity, affinity maturation rates, and functional validation of generated antibodies.

Theme
General food systems / other
Subject
Other / interdisciplinary
Study type
Research
Study design
Laboratory research / Methods development
Source type
Peer-reviewed study
Status
Published
Geography
United States
System type
Laboratory / in vitro
DOI
10.1038/s41589-021-00832-4
Catalogue ID
BFmobghs0w-6kugk3

Topic tags

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