Pulse Brain · Growing Health Evidence Index
Peer-reviewed

Genetically modified lentiviruses that preserve microvascular function protect against late radiation damage in normal tissues

Aadil Khan, James Paget, Martin McLaughlin, Joan Kyula, Michelle Wilkinson, Timothy Pencavel, David Mansfield, Victoria Roulstone, Rohit Seth, Martin Halle, Navita Somaiah, Jessica K.R. Boult, Simon P. Robinson, Hardev Pandha, Richard G. Vile, Alan Melcher, Paul A. Harris, Kevin J. Harrington

Science Translational Medicine · 2018

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Summary

Improvements in cancer survival mean that long-term toxicities, which contribute to the morbidity of cancer survivorship, are being increasingly recognized. Late adverse effects (LAEs) in normal tissues after radiotherapy (RT) are characterized by vascular dysfunction and fibrosis causing volume loss and tissue contracture, for example, in the free flaps used for immediate breast reconstruction after mastectomy. We evaluated the efficacy of lentivirally delivered superoxide dismutase 2 (SOD2) overexpression and connective tissue growth factor (CTGF) knockdown by short hairpin RNA in reducing the severity of LAEs in an animal model of free flap LAEs. Vectors were delivered by intra-arterial injection, ex vivo, to target the vascular compartment. LVSOD2 and LVshCTGF monotherapy before irradi

Subject
Other / interdisciplinary
Source type
Peer-reviewed study
System type
Other
DOI
10.1126/scitranslmed.aar2041
Catalogue ID
BFmoso8xrl-5jqsw7
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