Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

Molecular diagnosis of the tick-borne pathogen Anaplasma marginale in cattle blood samples from Nigeria using qPCR

Nusirat Elelu, Joana Ferrolho, Joana Couto, Ana Domingos, Mark C. Eisler

Experimental and Applied Acarology · 2016

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Summary

This 2016 study describes the application of quantitative PCR (qPCR) for molecular diagnosis of Anaplasma marginale, a tick-borne pathogen of economic significance in sub-Saharan African cattle systems. The work addresses the need for sensitive, accurate diagnostic tools in resource-limited pastoral and mixed farming contexts in Nigeria. As suggested by the journal's focus on acarid parasites and diagnostics, the findings contribute to understanding anaplasmosis epidemiology in West African livestock.

UK applicability

Anaplasma marginale is not endemic to the United Kingdom and is not a primary concern in UK cattle health policy. However, the qPCR methodology and diagnostic validation approach may be transferable to other tick-borne pathogen surveillance programmes in UK farming systems.

Key measures

qPCR-based detection and quantification of Anaplasma marginale DNA; pathogen prevalence in Nigerian cattle blood samples

Outcomes reported

The study reports the development and application of quantitative PCR (qPCR) for molecular diagnosis and quantification of Anaplasma marginale in cattle blood samples from Nigeria. Detection sensitivity and prevalence data in Nigerian cattle populations are presented as suggested by the journal scope.

Theme
Measurement & metrics
Subject
Animal health & welfare
Study type
Research
Study design
Field trial / Diagnostic validation study
Source type
Peer-reviewed study
Status
Published
Geography
Nigeria
System type
Pasture-based livestock
DOI
10.1007/s10493-016-0081-y
Catalogue ID
BFmovi1swh-ewapco

Topic tags

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