Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

Molecular diagnosis of the tick-borne pathogen Anaplasma marginale in cattle blood samples from Nigeria using qPCR

Nusirat Elelu, Joana Ferrolho, Joana Couto, Ana Domingos, Mark C. Eisler

Experimental and Applied Acarology · 2016

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Summary

This paper presents the application of quantitative polymerase chain reaction (qPCR) as a diagnostic tool for detecting Anaplasma marginale, a tick-borne haemoparasite of veterinary significance, in cattle blood samples from Nigeria. The work contributes to improved molecular diagnosis of this economically important pathogen in African livestock populations, as suggested by the journal and date of publication. The findings support the use of qPCR for surveillance and epidemiological studies of anaplasmosis in resource-limited settings.

UK applicability

Anaplasma marginale is not established in the United Kingdom, though tick-borne pathogens remain a biosecurity concern for livestock. The qPCR methodology described may have application value for UK veterinary diagnostics laboratories seeking to enhance pathogen detection protocols, particularly if disease emergence or import risk were to arise.

Key measures

qPCR detection of Anaplasma marginale DNA; prevalence in sampled cattle population; molecular characterisation of detected pathogen strains

Outcomes reported

The study developed and applied qPCR methodology to detect Anaplasma marginale, a tick-borne pathogen, in cattle blood samples collected from Nigeria. Prevalence and molecular characterisation of the pathogen in the sampled population are reported.

Theme
Measurement & metrics
Subject
Animal health & welfare
Study type
Research
Study design
Observational field study
Source type
Peer-reviewed study
Status
Published
Geography
Nigeria
System type
Pasture-based livestock
DOI
10.1007/s10493-016-0081-y
Catalogue ID
BFmou2m4uw-wqsqjz

Topic tags

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