Summary
This study examines the immobilisation of phytase sourced from Chenopodium murale onto three carrier materials — sodium alginate/PVA beads, cellulose beads, and glass microspheres — as a means of enhancing phytic acid hydrolysis in cereal-based foods. Glass microsphere carriers, employing covalent bonding, demonstrated superior catalytic performance, stability, and reusability compared with entrapment-based matrices. The findings suggest that carrier-immobilised plant-derived phytase may offer a practical and sustainable strategy for improving mineral bioavailability in staple grain products.
UK applicability
Whilst this study was not conducted in a UK context, its findings are broadly applicable to UK food processing and cereal manufacturing sectors, where reducing phytate-bound mineral losses is relevant to addressing dietary iron, zinc, and phosphorus bioavailability in plant-based and wholegrain food products.
Key measures
Phytase activity retention (%); rate constant K (min⁻¹); half-hydrolysis time τ50 (min); full hydrolysis time τ_complete (min); phytic acid hydrolysis efficiency; enzyme reusability across cycles
Outcomes reported
The study measured phytase catalytic activity, stability, and reusability across three carrier systems, assessing phytic acid hydrolysis kinetics in cereal-based food matrices. Key performance metrics including rate constants, half-hydrolysis time, and full hydrolysis time were reported for the optimised glass microsphere carrier.
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