Summary
This narrative review synthesises the historical trajectory and contemporary state of Cannabis sativa tissue culture, encompassing micropropagation techniques, organogenesis and somatic embryogenesis pathways, and applications in plant breeding and biotechnology. The authors examine technical optimisation challenges and project future directions for tissue culture-mediated improvement of cannabis cultivars, likely addressing both pharmaceutical and agronomic breeding objectives. The review positions tissue culture as a potentially valuable tool for standardisation and genetic advancement in an emerging commercial crop.
UK applicability
UK applicability is limited by current legal restrictions on cannabis cultivation, though the review may inform future regulatory and research frameworks should licencing expand. The tissue culture methodologies described could be relevant to licensed cultivation facilities, seed producers, and research institutions in the UK once legislative barriers are addressed.
Key measures
Tissue culture regeneration protocols, explant types, growth regulator combinations, regeneration efficiency, contamination rates, applications in clonal propagation and genetic modification
Outcomes reported
The paper reviews tissue culture methodologies for Cannabis sativa, synthesising historical development, current applications in plant propagation and breeding, and prospective biotechnological advances. It likely addresses technical challenges, scalability, and potential for genetic improvement in cannabis cultivation.
Topic tags
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