Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

Genotoxicity Assessment of Silver Nanoparticles Produced via HVAD: Examination of Sister Chromatid Exchanges in Chinchilla lanigera Blood Lymphocytes In Vitro

Anna Grzesiakowska, M. Kasprowicz, Olga Jarnecka, Marta Kuchta‐Gładysz

International Journal of Molecular Sciences · 2025

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Summary

This laboratory study assessed the genotoxic potential of silver nanoparticles (AgNPs) produced via the HVAD method by exposing chinchilla blood lymphocytes to three colloidal silver compounds and measuring chromosome damage via sister chromatid exchange analysis. The work reveals that citrate-stabilised silver nanoparticles induced the highest level of chromosome damage, with all tested compounds showing genotoxic properties. The findings suggest production method and stabilisation approach significantly influence AgNP toxicity in mammalian cells, though further mechanistic research is needed to understand HVAD-produced AgNPs specifically.

UK applicability

This in vitro genotoxicity assessment provides foundational evidence relevant to UK regulation and risk assessment of silver nanoparticles in consumer and industrial products. The findings may inform occupational health guidelines and product safety standards in the UK, though application to human exposure requires additional in vivo validation.

Key measures

Sister chromatid exchange (SCE) frequency in mitotic chromosomes; exposure concentrations of 5, 10, and 20 µg/L; exposure durations of 3, 6, and 24 hours

Outcomes reported

The study measured sister chromatid exchanges (SCEs) in chinchilla blood lymphocytes exposed to three colloidal silver compounds at varying concentrations and exposure durations. Results demonstrated a notable increase in SCEs compared to control groups, indicating genotoxic properties of the tested silver nanoparticles.

Theme
Measurement & metrics
Subject
Pesticides, contaminants & food safety
Study type
Research
Study design
Laboratory in vitro experimental study
Source type
Peer-reviewed study
Status
Published
Geography
Poland
System type
Laboratory / in vitro
DOI
10.3390/ijms262311703
Catalogue ID
SNmoi1qb4u-gbb9pb

Topic tags

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