Pulse Brain · Growing Health Evidence Index
Tier 4 — Narrative / commentaryPeer-reviewed

Advances in the isolation and characterization of milk-derived extracellular vesicles and their functions

Shujuan Di, Yibo Huang, Weicang Qiao, Xiaomei Zhang, Yaling Wang, Minghui Zhang, Jiao Fu, Junying Zhao, Lijun Chen

Frontiers in Nutrition · 2024

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Summary

This review consolidates current knowledge on milk-derived extracellular vesicles, focusing on isolation and characterisation methodologies essential for functional and omics studies. The paper synthesises evidence demonstrating that milk-derived EVs carry bioactive molecules (proteins, lipids, nucleic acids) and mediate inter-cellular signalling, with established roles in immune regulation and intestinal development. The authors argue that rigorous selection of isolation and characterisation methods is prerequisite for downstream research and development of these vesicles for application in infant nutrition and functional foods.

UK applicability

Findings on milk-derived EV isolation and characterisation are methodologically applicable to UK dairy research and infant formula development, particularly relevant to UK food safety and nutrition standards for functional foods. However, the abstract does not specify UK-specific regulatory or production contexts.

Key measures

Methods for EV isolation and characterisation; structural integrity and biological viability metrics; functional outcomes including immune regulation and intestinal development promotion

Outcomes reported

This review summarises isolation and characterisation methods for milk-derived extracellular vesicles (EVs) and their biological functions. The paper synthesises evidence on the roles of milk-derived EVs in immune regulation and intestinal development, and their potential applications in infant formula and functional food development.

Theme
Nutrition & health
Subject
Maternal, infant & child nutrition
Study type
Narrative Review
Study design
Narrative review
Source type
Peer-reviewed study
Status
Published
System type
Dairy
DOI
10.3389/fnut.2024.1512939
Catalogue ID
SNmojmgqqt-soyfgt

Topic tags

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