Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

Chromosome-scale genome assembly and de novo annotation of Alopecurus aequalis

Jonathan Wright, Kendall Baker, Tom Barker, Leah Catchpole, Alex Durrant, F C Fraser, Karim Gharbi, Christian Harrison, S. M. Henderson, Naomi Irish, Gemy Kaithakottil, Ilia J. Leitch, Jun Li, Sacha Lucchini, Paul Neve, Robyn F. Powell, Hannah Rees, David Swarbreck, Christopher B. Watkins, Jonathan Wood, Seanna McTaggart, Anthony Hall, Dana R. MacGregor

Scientific Data · 2024

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Summary

This paper presents a chromosome-scale reference genome assembly for Alopecurus aequalis, an increasingly problematic winter annual bunchgrass weed in barley and wheat crops across China and Japan that displays significant herbicide resistance. The assembled genome, containing over 33,000 annotated genes, provides a foundational genomic resource for understanding the weed's biology and resistance mechanisms. The comparative analysis suggests greater evolutionary affinity with cultivated barley than with a morphologically closer relative, potentially informing future research into herbicide resistance mechanisms and weed management strategies.

UK applicability

Whilst A. aequalis is not currently a major agricultural weed in the United Kingdom, this genomic resource may prove valuable if the species expands its range northward or if UK farmers encounter related herbicide-resistant grass weed species. The methodological approach and insights into herbicide resistance evolution could inform UK-based weed genomics research.

Key measures

Genome size (2.83 Gb); number of protein-coding genes (33,758); comparative genomic structure analysis

Outcomes reported

The study reports a chromosome-scale genome assembly for Alopecurus aequalis with a genome size of 2.83 Gb and 33,758 high-confidence protein-coding genes with functional annotation. Comparative genomics analysis revealed structural similarity between A. aequalis and barley (Hordeum vulgare) rather than the more closely related A. myosuroides.

Theme
Farming systems, soils & land use
Subject
Cereals & grains
Study type
Research
Study design
Laboratory / genomic resource development
Source type
Peer-reviewed study
Status
Published
Geography
International
System type
Arable cereals
DOI
10.1038/s41597-024-04222-y
Catalogue ID
SNmok3j3w8-v5f0hs

Topic tags

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