Summary
cultures, supporting the identification of shared metabolites linked to active methanogenesis. Extended 60-day incubations confirmed temperature-sensitive CH₄ production. Carbon isotopic enrichment in CH₄ was unexpectedly strong with warming, and metagenomic detection of ANME-associated markers-including multiheme cytochromes and formate dehydrogenases-supports temperature-sensitive anaerobic oxidation of methane as a significant control on isotopic signatures. Calculated Q₁₀ values for methanogenesis exceeded typical values for boreal soils, highlighting an underappreciated temperature responsiveness of Arctic methanogens. Together, these results demonstrate that VOCs can serve as informative biomarkers of subsurface microbial activation and offer a novel diagnostic tool for detecting ear
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