Pulse Brain · Growing Health Evidence Index
Tier 4 — Narrative / commentaryPeer-reviewed

Progress in serology and molecular biology of equine parasite diagnosis: sustainable control strategies.

Wang T, Chen X, Yan X, Su Y, Gao W, Liu C, Wang W.

Front Vet Sci · 2025

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Summary

This narrative review synthesises recent advances in serological and molecular biological diagnostic methods for equine parasites, with emphasis on gene-based identification techniques for strongylids and related taxa. The authors note that whilst laboratory-based diagnostic methods have proliferated, few have transitioned to practical field use, and argue that whole genome sequencing and high-throughput technologies offer promising pathways to develop clinically applicable diagnostics that could support sustainable parasite control and mitigate antimicrobial resistance.

UK applicability

UK horse owners and veterinary practitioners face endemic equine parasite challenges and emerging anthelmintic resistance; improved diagnostic methods reviewed here could support targeted treatment protocols and inform evidence-based grazing management on UK pastures, though implementation barriers and cost-effectiveness in field settings remain unaddressed.

Key measures

Diagnostic sensitivity and specificity of serological methods (ELISA); molecular markers (ITS1, ITS2, COI, IGS); whole genome sequencing data availability; applicability to real-time field diagnostics

Outcomes reported

The review examines progress in serological and molecular biological diagnostic techniques for major equine parasites, evaluating their current application and limitations. It assesses the potential of genomic sequencing and high-throughput technologies to develop more precise field-applicable diagnostic tools.

Theme
Measurement & metrics
Subject
Animal health & welfare
Study type
Narrative Review
Study design
Narrative review
Source type
Peer-reviewed study
Status
Published
System type
Pasture-based livestock
DOI
10.3389/fvets.2025.1663577
Catalogue ID
MGmob8kmzy-1vepk5

Topic tags

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