Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

High Intensity Interval Training in Aged Female Mice Preserves Physical, Cognitive, and Cardiovascular Function

Theobald, D.; Williamson, P.; Johnston, A.; Tripp, L.; Olabiyi, A. A.; Silvers, X.; Dickerson, A.; Tran, T. D.; de Castro Braz, L.; Sriramula, S.; Graber, T. G.

bioRxiv · 2026

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Summary

This controlled laboratory study demonstrates that 14 weeks of progressive high-intensity interval training significantly attenuated age-related declines across physical, cognitive, and cardiovascular domains in aged female C57BL/6 mice. HIIT increased aerobic capacity by 71%, preserved or improved strength and motor function, enhanced executive function by 73%, and reduced systolic and mean arterial blood pressure. The intervention also reduced cardiac fibrosis and hypothalamic inflammation whilst mitigating frailty onset.

Regional applicability

This is a preclinical mouse study and does not directly translate to human populations or United Kingdom agricultural or farming systems. The findings may inform future intervention development for age-related functional decline in humans, but require validation in human clinical trials.

Key measures

Aerobic capacity (% change), four-limb strength/endurance (% decline), forelimb strength, motor function (% improvement), executive function (% improvement), systolic blood pressure (mmHg), mean arterial pressure (mmHg), cardiac fibrosis, muscle fibre type 2a percentage, IL-1β expression in hypothalamus, frailty onset

Outcomes reported

The study measured physical function (aerobic capacity, strength, motor control), cognitive function (executive function, exploratory behaviour), and cardiovascular function (blood pressure, cardiac structure) in aged female mice following 14 weeks of high-intensity interval training versus sedentary control.

Theme
Nutrition & health
Subject
Out of scope / non-food
Study type
Research
Study design
Controlled laboratory trial
Source type
Peer-reviewed study
Status
Published
Geography
United States
System type
Laboratory / in vitro
DOI
10.64898/2026.06.07.730494
Catalogue ID
IR-ESmqhcvjzv-f50ec0

Topic tags

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