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Tier 3 — Observational / field trialPreprintConventional

Anthropogenic disturbance inverts microclimate stratification in tropical forests

Nunes, C. A.; Berenguer, E.; do Nascimento, R. O.; Martins, R. G.; Metcalf, O. C.; Lees, A. C.; Smith, M. N.; Ferreira, J.; Maclean, I.; Barlow, J.

bioRxiv · 2026

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Summary

Tropical rainforests generate and maintain their own microclimate regimes and the resultant cooler, more humid and stable environments foster the hyperdiversity typical of these ecosystems. Although the temporal and spatial (horizontal) distributions of microclimates have been relatively well studied, the vertical dimension has received less attention, and little is known about how forest disturbance affects the vertical stratification of microclimates in tropical forests. In this study, we examine how the vertical distribution of temperatures varies between undisturbed and burned Amazonian forests. We installed five vertical transects with temperature dataloggers distributed at 7 different heights to collect data over multiple days during the end of the dry season. We investigated how anthropogenic disturbance (fire) mediates the vertical stratification of microclimate and whether microclimate buffering (i.e, the difference between understorey and canopy temperatures) varies according to the forest structure. We showed that anthropogenic disturbance can cause an inversion in the vertical stratification of microclimates, with burned forests having hotter temperatures (up to 2 degrees C) in the understorey than in the canopy during the day - the opposite of what is found in undisturbed forests (typically 3 degrees C cooler). During the night, while understorey and canopy temperatures are similar in undisturbed forests, we found that, in burned forests, understorey temperatures were up to 2 degrees C cooler than in the canopy. Microclimate buffering by day was best explained by aboveground carbon stocks, with higher temperature buffering in more carbon rich forests. Our study shows that anthropogenic disturbance alters the vertical stratification of temperatures in Amazonian forests, leading to significant temporal changes along the diel cycle. Future research should focus on understanding these changes across a wider range of disturbance regimes, and explore the consequences for biodiversity and ecosystem functions from the canopy to the forest floor.

Outcomes reported

Tropical rainforests generate and maintain their own microclimate regimes and the resultant cooler, more humid and stable environments foster the hyperdiversity typical of these ecosystems. Although the temporal and spatial (horizontal) distributions of microclimates have been relatively well studied, the vertical dimension has received less attention, and little is known about how forest disturbance affects the vertical stratification of microclimates in tropical forests. In this study, we examine how the vertical distribution of temperatures varies between undisturbed and burned Amazonian forests. We installed five vertical transects with temperature dataloggers distributed at 7 different heights to collect data over multiple days during the end of the dry season. We investigated how anthropogenic disturbance (fire) mediates the vertical stratification of microclimate and whether microclimate buffering (i.e, the difference between understorey and canopy temperatures) varies according to the forest structure. We showed that anthropogenic disturbance can cause an inversion in the vertical stratification of microclimates, with burned forests having hotter temperatures (up to 2 degrees C) in the understorey than in the canopy during the day - the opposite of what is found in undisturbed forests (typically 3 degrees C cooler). During the night, while understorey and canopy temperatures are similar in undisturbed forests, we found that, in burned forests, understorey temperatures were up to 2 degrees C cooler than in the canopy. Microclimate buffering by day was best explained by aboveground carbon stocks, with higher temperature buffering in more carbon rich forests. Our study shows that anthropogenic disturbance alters the vertical stratification of temperatures in Amazonian forests, leading to significant temporal changes along the diel cycle. Future research should focus on understanding these changes across a wider range of disturbance regimes, and explore the consequences for biodiversity and ecosystem functions from the canopy to the forest floor.

Theme
Farming systems, soils & land use
Subject
Other / interdisciplinary
Study type
Research
Source type
Preprint
Status
Preprint
Geography
United Kingdom
System type
Other
DOI
10.64898/2026.06.11.731463
Catalogue ID
IR-ESmqhcvk6q-c471e3
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