Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewedConventional

Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts

Kriengsak Thaipong, Unaroj Boonprakob, Kevin M. Crosby, Luis Cisneros‐Zevallos, David Byrne

Journal of Food Composition and Analysis · 2006

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Summary

This 2006 study evaluated the comparative performance of four widely-used antioxidant capacity assays (ABTS, DPPH, FRAP, and ORAC) applied to guava fruit extracts. By systematically assessing these measurement methods, the work provides methodological evidence for standardising antioxidant evaluation in fruit-based research and quality assessment. The findings likely inform which assay best suits different research objectives in fruit antioxidant profiling.

Regional applicability

Guava is not a primary commercial crop in the United Kingdom climate; however, the methodological comparison of antioxidant assays is directly applicable to UK horticulture research on native and cultivated fruits (e.g., apples, berries, stone fruits). The standardisation framework from this study could inform UK-based phytochemical evaluation protocols.

Key measures

Antioxidant activity measured by ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (Ferric Reducing Antioxidant Power), and ORAC (Oxygen Radical Absorbance Capacity) assays

Outcomes reported

The study compared four different spectrophotometric assays (ABTS, DPPH, FRAP, and ORAC) for measuring antioxidant activity in guava fruit extracts. The work evaluated the concordance and practical utility of these measurement methods.

Theme
Measurement & metrics
Subject
Measurement methods & nutrient profiling
Study type
Research
Study design
Laboratory comparison study
Source type
Peer-reviewed study
Status
Published
Geography
United States
System type
Horticulture
DOI
10.1016/j.jfca.2006.01.003
Catalogue ID
SNmqq4q1bn-yavxet

Topic tags

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