Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewedConventional

Environmental DNA analysis to search for an endangered desert fish

Fulkerson, A.; Gutierrez-Barragan, A.; Varela-Romero, A.; Duncan, D. K.; Mateos, M.

bioRxiv · 2026

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Summary

This field survey applied environmental DNA metabarcoding to search for an endangered microendemic desert fish (Rio Concepcion Topminnow) across Mexican freshwater habitats. Despite eDNA analysis and visual surveys over two consecutive years, the target species was not detected, suggesting either extinction or populations below detection thresholds. The study documents the presence of native species and recent invasive fish introductions whilst discussing methodological limitations of eDNA approaches in conservation monitoring.

Regional applicability

This study was conducted in Mexico and addresses Mexican desert fish conservation. Whilst the eDNA metabarcoding methodology may be transferable to United Kingdom freshwater surveys, the findings are specific to Mexican fish fauna and water system management challenges (groundwater recharge, wastewater treatment) distinct from UK contexts.

Key measures

Detection/absence of target fish species using eDNA metabarcoding; presence of native and introduced teleost species; detection limitations of eDNA approach

Outcomes reported

The study used eDNA metabarcoding (MiFish locus) to survey freshwater habitats in Rio De La Concepcion and Rio Santa Cruz in Sonora, Mexico over two years, failing to detect the endangered Rio Concepcion Topminnow (Poeciliopsis jackschultzi) but identifying native species and documenting invasive fish introductions.

Theme
Farming systems, soils & land use
Subject
Aquaculture & fisheries
Study type
Research
Study design
Field survey
Source type
Peer-reviewed study
Status
Preprint
Geography
Mexico
System type
Aquaculture
DOI
10.64898/2026.05.20.726406
Catalogue ID
IRmpspqbmi-70f3ff

Topic tags

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