Summary
Epimedium sagittatum bee pollen (EBP) is a bee pollen product of a medicinal plant, but its chemical characteristics remain unclear. In this study, EBP was systematically characterized by biochemical analysis and UPLC-MS/MS, using Brassica rapa bee pollen (BBP), Camellia sinensis bee pollen (CBP), and Epimedium leaves for compari-son. A total of 1,073 secondary metabolites were identified in EBP, mainly flavonoids (330, 30.8%) and phenolic acids (165, 15.3%). EBP showed the highest total flavonoid content among the three bee pollen types (4.75 mg/g) and was clearly separated from BBP and CBP in multivariate analysis. EBP contained 19 unique metabolites, fewer than CBP (271), but these included several high-content flavonoid compounds, such as cacticin, brassicin, and tricetin-4'-methyl ether-3'-{beta}-D-glucoside. Differential metabolite analysis identified 449 and 1,085 metabolites that differed between EBP and BBP, re-spectively, with flavonoid compounds forming the main differential class. Among the shared differential metabolites, 45 flavonoids were consistently higher in EBP, in-cluding kaempferol, tamarixetin, and sinensetin. Network pharmacology screening further suggested that flavonoid metabolites, especially kaempferol, tamarixetin, and sinensetin, deserve particular attention in future studies of EBP. Compared with Epimedium leaves, characteristic leaf flavonoids such as icariin and epimedins A-C were present at very low levels or were not detected in EBP. Overall, EBP is charac-terized by a distinct, relatively concentrated flavonoid chemical profile, distinct from both common commercial bee pollens and Epimedium medicinal tissues. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=160 SRC="FIGDIR/small/679240v2_ufig1.gif" ALT="Figure 1"> View larger version (56K): org.highwire.dtl.DTLVardef@5973eborg.highwire.dtl.DTLVardef@c953bcorg.highwire.dtl.DTLVardef@1989ba5org.highwire.dtl.DTLVardef@127081_HPS_FORMAT_FIGEXP M_FIG C_FIG
Outcomes reported
Epimedium sagittatum bee pollen (EBP) is a bee pollen product of a medicinal plant, but its chemical characteristics remain unclear. In this study, EBP was systematically characterized by biochemical analysis and UPLC-MS/MS, using Brassica rapa bee pollen (BBP), Camellia sinensis bee pollen (CBP), and Epimedium leaves for compari-son. A total of 1,073 secondary metabolites were identified in EBP, mainly flavonoids (330, 30.8%) and phenolic acids (165, 15.3%). EBP showed the highest total flavonoid content among the three bee pollen types (4.75 mg/g) and was clearly separated from BBP and CBP in multivariate analysis. EBP contained 19 unique metabolites, fewer than CBP (271), but these included several high-content flavonoid compounds, such as cacticin, brassicin, and tricetin-4'-methyl ether-3'-{beta}-D-glucoside. Differential metabolite analysis identified 449 and 1,085 metabolites that differed between EBP and BBP, re-spectively, with flavonoid compounds forming the main differential class. Among the shared differential metabolites, 45 flavonoids were consistently higher in EBP, in-cluding kaempferol, tamarixetin, and sinensetin. Network pharmacology screening further suggested that flavonoid metabolites, especially kaempferol, tamarixetin, and sinensetin, deserve particular attention in future studies of EBP. Compared with Epimedium leaves, characteristic leaf flavonoids such as icariin and epimedins A-C were present at very low levels or were not detected in EBP. Overall, EBP is charac-terized by a distinct, relatively concentrated flavonoid chemical profile, distinct from both common commercial bee pollens and Epimedium medicinal tissues. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=160 SRC="FIGDIR/small/679240v2_ufig1.gif" ALT="Figure 1"> View larger version (56K): org.highwire.dtl.DTLVardef@5973eborg.highwire.dtl.DTLVardef@c953bcorg.highwire.dtl.DTLVardef@1989ba5org.highwire.dtl.DTLVardef@127081_HPS_FORMAT_FIGEXP M_FIG C_FIG
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