Pulse Brain · Growing Health Evidence Index
Tier 1 — Meta-analysis / systematic reviewPeer-reviewedConventional

Species mixing promotes plant biomass accumulation and nutrient cycling in forest plantations

Hui Zhang; Huili Feng; Xinjing Qu; Mei Yang; Mengke Huang; Douglass Frederick Jacobs; Jiahuan Guo

Communications Biology · 2026

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Summary

This global meta-analysis of 8,450 paired observations from 328 studies demonstrates that mixed-species forest plantations significantly enhance plant biomass accumulation and nutrient cycling relative to monocultures across diverse climatic zones and stand structures. The beneficial effects of species mixing were modulated by environmental context, with stronger outcomes in warmer, wetter climates and increasing with species richness, whilst showing unimodal relationships with elevation, stand age and density. These findings support mixed-species plantations as a silvicultural approach to improve ecosystem function and support forest restoration.

Regional applicability

Findings are geographically global in scope. Application to United Kingdom conditions would be most relevant for temperate maritime climates; the meta-analysis indicates moderate effects in cooler climates compared to warmer zones, so UK plantation managers should expect benefits of species mixing but potentially smaller magnitude than in tropical or subtropical contexts.

Key measures

Plant biomass (trees, shrubs, litterfall, above- and belowground compartments); soil organic carbon; total nitrogen; phosphorus availability; microbial biomass; leaf nutrient content; soil stoichiometric ratios

Outcomes reported

The study quantified the effects of species mixing on plant biomass accumulation, soil nutrient content (carbon, nitrogen, phosphorus), microbial biomass, and leaf nutrient composition compared to monoculture plantations. Effects were examined across multiple ecosystem compartments and examined how outcomes vary by climate, elevation, stand age, species richness and stand density.

Theme
Farming systems, soils & land use
Subject
Soil carbon & organic matter
Study type
Meta-analysis
Study design
Meta-analysis
Source type
Peer-reviewed study
Status
Published
Geography
Global
System type
Agroforestry
DOI
10.1038/s42003-026-09646-3
Catalogue ID
NRmucqvdig-006

Topic tags

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