Summary
In this study, we evaluated zinc (Zn) and selenium (Se) biofortification in strawberry fruits under substrate and soil cultivation, along with their effects on mineral element accumulation and fruit quality. To achieve this, foliar Zn (0.1% and 0.2%) and Se (0.003% and 0.006%) fertilizers were applied separately or in combination at the initial flowering stage. The Zn and Se contents in strawberry fruits increased with the spraying dosage. Compared to the control, the Zn content in the first batch of Zn-treated strawberries increased by 36.9–109% and 27.1–102% under substrate and soil cultivation, respectively, while Se increased by 313–444% and 21.3–53.3%, respectively. However, foliar Zn application could not ensure long-term sustainability as Zn in strawberries gradually decreased in th
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