Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

Optimization of nutrient medium for DH cucumber lines from ovules

Belov S, Korottseva I, Domblides E

In Vitro Cellular & Developmental Biology - Plant · 2025

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Summary

This laboratory study addresses the optimisation of nutrient medium formulations required for efficient regeneration of doubled haploid cucumber lines from cultured ovules. Doubled haploid breeding is a rapid method for producing homozygous lines in crop improvement programmes. The work is likely to be of technical interest to plant breeders and horticultural research programmes seeking to accelerate cultivar development in cucumbers.

Regional applicability

Whilst not UK-based (authors and journal suggest a European or international collaboration), the methodology and findings would be directly transferable to UK horticulture research and commercial cucumber breeding programmes, particularly for those adopting accelerated breeding strategies.

Key measures

Nutrient medium composition variables, regeneration rate, plant recovery frequency, tissue culture success metrics

Outcomes reported

The study likely reports optimised nutrient medium composition and culture conditions for generating doubled haploid (DH) cucumber lines via ovule-derived tissue culture. Outcome measures presumably include regeneration frequency, plant survival rates, and characteristics of recovered lines.

Theme
Farming systems, soils & land use
Subject
Measurement methods & nutrient profiling
Study type
Research
Study design
Laboratory / in vitro experimental design (tissue culture optimisation study)
Source type
Peer-reviewed study
Status
Published
System type
Horticulture
DOI
10.1007/s11627-025-10598-2
Catalogue ID
BImvhai1ka-omwlnt

Topic tags

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