Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewedRegenerative

Microbial and Metabolite Profiling Reveal the Composition of Beejamrit: A Bioformulation for Seed Treatment in Sustainable Agriculture

Devarsh Panchal; Kartik Gajjar; Mahendra Chaudhary; D. Chaudhary; C. Patel; Nitin Shukla; Ishan Raval; Snehal Bagatharia; Chaitanya G. Joshi; Amrutlal K. Patel; D. Dharajiya

Agriculture · 2026

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Summary

This study employed metagenomics and untargeted metabolomics to characterise the composition of Beejamrit, a traditional Indian cow-based biostimulant used for seed treatment in natural farming systems. Analysis revealed the presence of plant-growth-promoting bacteria (including Advenella, Comamonas, Lysinibacillus, Acinetobacter and Arcobacter) and diverse bioactive metabolites, with organoheterocyclics (23–18%), organic acids (18%) and lipids (26%) as predominant compounds. These findings provide mechanistic insight into Beejamrit's potential efficacy and suggest a framework for standardisation in sustainable agriculture practice.

Regional applicability

Beejamrit is a traditional practice in India and this characterisation is most directly applicable to Indian natural farming systems. The study's findings on microbial and metabolite composition could inform efforts to standardise and validate bioformulations in other agroecological contexts, though the cow-based formulation reflects specific cultural and agricultural practices in South Asia.

Key measures

16S rRNA amplicon metagenomics; GC-MS metabolite profiling; LC-MS metabolite profiling; bacterial taxa composition; metabolite class abundance (organoheterocyclics, organic acids, benzenoids, lipids, phenolics, amino acids, fatty acids)

Outcomes reported

The study characterised the microbial composition and metabolite profiles of Beejamrit samples using 16S rRNA metagenomics, GC-MS and LC-MS analyses. It identified plant-growth-promoting bacteria and a diverse array of bioactive metabolites including amino acids, organic acids, phenolics and fatty acids.

Theme
Farming systems, soils & land use
Subject
Regenerative & agroecological farming
Study type
Research
Study design
Laboratory / in vitro analytical study
Source type
Peer-reviewed study
Status
Published
Geography
India
System type
Organic systems
DOI
10.3390/agriculture16010133
Catalogue ID
NRmupdc5m3-0aw

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