Summary
This study describes the development of a dual-mode lateral flow immunoassay for rapid, sensitive detection of acetamiprid residues in plant-based foods using trimetallic nanoparticles as signal probes. The assay operates in both colorimetric and photothermal modes, achieving submicrogram-per-litre detection limits and demonstrating good agreement with conventional analytical methods (HPLC, ELISA) and high recovery rates in spiked food matrices. The technology offers a portable, field-applicable tool for neonicotinoid pesticide monitoring in food systems.
Regional applicability
The study does not specify a geographic location and presents a general analytical methodology. The approach could be applicable to United Kingdom food safety testing and regulatory monitoring for acetamiprid residues in imported and domestic plant-based foods, though UK-specific validation against native produce matrices and regulatory thresholds would be needed.
Key measures
Detection limit (μg/L), linear detection range (0.1–3000 μg/L), inhibition rate, temperature measurement via smartphone thermal imager, cross-reactivity rate (%), recovery rate (%), relative standard deviation (RSD, %), consistency with HPLC and ELISA
Outcomes reported
The study developed and validated a dual-mode lateral flow immunochromatographic assay (DMLFIA) using Au@Ag@Pt nanoparticles for detecting acetamiprid residues in plant-based foods. The assay achieved detection limits of 0.08 μg/L (colorimetric mode) and 0.1 μg/L (photothermal mode) with recovery rates of 81.1–99.4% in spiked food samples.
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