Summary
This study describes the development of a polarity-responsive fluorescent probe (AFA) designed to image lipid droplets in foam cells and assess cellular oxidative stress relevant to intracranial atherosclerosis. The probe exhibits solvent-dependent solvatochromism, remaining optically stable and biocompatible whilst selectively localising to lipid droplets with fluorescence intensity correlating to oxidative stress levels. The authors demonstrate the probe's practical utility by screening six bioactive compounds from Salvia miltiorrhiza, suggesting potential application in identifying cerebroprotective natural products.
Regional applicability
This is a fundamental laboratory study with no specific geographical focus or agricultural/farming system element. The findings are potentially applicable globally to biomedical research on atherosclerosis and natural product screening, but do not directly address United Kingdom farming systems, soil health, or food production.
Key measures
Fluorescence emission wavelength (592 nm), optical stability duration (80+ min), pH range effectiveness (6.0–11.0), cytotoxicity, biocompatibility, lipid droplet localisation, fluorescence intensity correlation with Ox-LDL levels and intracellular oxidative stress
Outcomes reported
Development and validation of a polarity-responsive fluorescent probe (AFA) that selectively targets lipid droplets in foam cells and enables screening of anti-atherosclerotic bioactive compounds from Salvia miltiorrhiza. The probe's fluorescence intensity correlates with lipid droplet accumulation and cellular oxidative stress in Ox-LDL-induced foam cells.
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