Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewed

Formulation and Stability Screening of Walnut Protein Concentrate-Whey Protein Isolate Stabilized Pickering Emulsions with Different Compounding Ratios: Capsanthin Loading, Multi-Condition Stability, and Application as a Butter Replacer in Spreadable Products.

Sawaidul M, Li J, Xu M, Zhang J, Zhang T, Meng X, Ma Y.

Foods (Basel, Switzerland) · 2026

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Summary

This study developed and optimised dual-protein Pickering emulsions using walnut protein concentrate and whey protein isolate as stabilisers for delivery of the bioactive compound capsanthin and functional fat replacement. A WPI-WalPC ratio of 1:9 demonstrated superior emulsifying properties and stability across multiple environmental stresses (storage, light, thermal, high-pressure). When incorporated as a 30% butter replacement in spreadable products, the optimised capsanthin-loaded emulsion improved sensory acceptability and oxidative stability while reducing acid and peroxide values, suggesting potential for development of nutritionally enhanced spreadable foods.

Regional applicability

This laboratory study on protein-stabilised emulsion technology and functional ingredient delivery has potential application in United Kingdom food product development, particularly for reformulation of spreads and other lipophilic delivery systems. Transferability depends on regulatory status of walnut protein concentrate and capsanthin as food ingredients in UK/EU markets, and sensory/oxidative stability data would require validation in commercial spreadable product contexts.

Key measures

Emulsifying activity index (EAI, m²/g), emulsifying stability index (ESI, min), capsanthin retention (%), acid value, peroxide value, sensory acceptability scores

Outcomes reported

The study evaluated functional properties and stability of dual-protein Pickering emulsions at different WPI-WalPC ratios, and assessed their performance as capsanthin delivery systems and butter replacers in spreadable products. Optimal formulations were tested for capsanthin retention under multiple storage conditions and sensory/oxidative stability when incorporated into spreadable pastes.

Theme
Nutrition & health
Subject
Food processing & bioavailability
Study type
Research
Study design
Laboratory / in vitro formulation and stability screening
Source type
Peer-reviewed study
Status
Published
System type
Food supply chain
DOI
10.3390/foods15193427
Catalogue ID
NRmvfomql3-009

Topic tags

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