Pulse Brain · Growing Health Evidence Index
Tier 3 — Observational / field trialPeer-reviewedConventional

Bioactive Silages from Agro-Industrial By-Products Based on Grape Pomace or Olive Mill Wastewater for Ruminants: Evolution of Phenolic Profiles, Antioxidant Activity, and Fatty Acid Composition

R. Di Benedetto; M. Sánchez-Parra; J. Ordóñez-Díaz; A. Di Luca; G. Martemucci; J. Moreno-Rojas; A. D'Alessandro

Antioxidants · 2026

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Summary

This experimental study compared two mixed silages formulated from wheat straw, cheese-whey, and molasses combined with either grape pomace or olive mill wastewater as ruminant feed. Silage incorporating grape pomace demonstrated superior nutritional quality, with higher crude protein, favourable fatty-acid composition, greater bioactive phenolic content, and improved pH stability over the 150-day ensiling period. The findings suggest grape pomace-based silage has potential as a functional feed ingredient whilst valorising agro-industrial by-products.

Regional applicability

The study geography is not specified in the metadata provided. The methodology is applicable to United Kingdom livestock farming, particularly given the availability of dairy by-products (whey) and potential access to Mediterranean agro-industrial wastes via supply chains; however, local availability of grape pomace and olive mill wastewater would be the primary constraint to adoption in UK practice.

Key measures

Crude protein content, dry matter, structural fiber fractions, pH, buffering capacity, short-chain fatty acids, lactic acid concentration, total phenolic content, antioxidant capacity, polyphenolic profiles, long-chain fatty acids

Outcomes reported

The study measured chemical composition, fermentation dynamics, fatty-acid profiles, and polyphenolic evolution during 150-day ensiling of two mixed silages incorporating grape pomace or olive mill wastewater. Both silages were evaluated for their nutritional quality, bioactive compound retention, and suitability as ruminant feed.

Theme
Nutrition & health
Subject
Livestock nutrition & meat quality
Study type
Research
Study design
Field trial
Source type
Peer-reviewed study
Status
Published
System type
Pasture-based livestock
DOI
10.3390/antiox15060692
Catalogue ID
NRmvpkinwc-009

Topic tags

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