Pulse Brain · Growing Health Evidence Index
Tier 4 — Narrative / commentaryPeer-reviewed

Chlorophylls and Carotenoids: Measurement and Characterization by UV ‐ VIS Spectroscopy

Hartmut K. Lichtenthaler, Claus Buschmann

Current Protocols in Food Analytical Chemistry · 2001

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Summary

This is a methodological protocol for measuring chlorophylls and carotenoids using UV-VIS spectroscopy, published in the Current Protocols series. The work provides standardised procedures likely intended for food analysis and quality assessment. As a methods paper from 2001, it represents established analytical techniques for quantifying key plant pigments that relate to both nutritional value and food quality.

Regional applicability

The spectroscopic methodology is geographically neutral and widely applicable; such protocols have become standard in UK food laboratories and research institutions for nutrient profiling and quality control across horticulture and processed food sectors.

Key measures

Chlorophyll a, chlorophyll b, xanthophyll, carotene concentrations and ratios measured by UV-VIS spectroscopy

Outcomes reported

This protocol paper describes standardised UV-VIS spectroscopic methods for quantifying and characterising chlorophylls and carotenoids in food samples. The methods enable rapid measurement of photosynthetic pigment composition and concentration.

Theme
Measurement & metrics
Subject
Measurement methods & nutrient profiling
Study type
Guideline
Study design
Guideline
Source type
Peer-reviewed study
Status
Published
System type
Laboratory / in vitro
DOI
10.1002/0471142913.faf0403s01
Catalogue ID
SNmv1wu566-hb0vqr

Topic tags

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